The Application of Chromosome Set Manipulations and the Importance of Gamete Collection and Management in Aquaculture
Chromosome Set Manipulation (CSM) technology is used in fish and shellfish aquaculture and has important applications in the development of unique genotypes for basic research (haploid, meiotic and mitotic gynogenetic and androgenetic individuals) and direct commercial applications to the aquaculture industry (sterile triploids and investigation and manipulation of sex-determination systems e.g. YY males). The workshop will give practical training in the collection, handling and storage of gametes for these purposes, from a range of species, but with a focus on salmonids and tilapias as examples. The course will compare and contrast the range of shock treatments that have been used to enhance the retention of the second meiotic and first mitotic divisions across species. The UV and X-ray inactivation of milt and ova DNA will be undertaken, and the optimisation of such procedures discussed. Methods for validation of the offspring from the various experiments will be discussed and a range of techniques (e.g. karyotyping, flow cytometry, genetic fingerprinting, sex ratio) demonstrated. Tilapia and trout will be used for the practical work but the teaching outcome should enable those completing the course to develop a programme of spawning induction, gamete management and CSM for research or commercial purposes in a new fish species. The course will use the experiences, examples and data generated through Work Package 9 (WP9) of the AQUAEXCEL project.
Main parts of the course:
- Principles of CSM in aquatic organisms
- Applications of CSM in the management of aquatic organisms
- Gamete management
- Applications of gamete storage
- Tilapia reproductive biology
- Trout reproductive biology
- Cool storage and cryopreservation
- Application of technologies
- Gynogenesis and androgenesis
- Double haploids and isogenic lines
- Verification of ploidy status
- Aquaculture and restocking
Institute of Aquaculture (IoA), University of Stirling, with additional inputs from scientists based at l’Institut National de la Recherche Agronomique (INRA), France, and the Institute of Marine Research (IMR), Norway
Participants should have some experience in the handling of live fish and want to gain experience in gamete handling and management and the application of CSM techniques in the course of their work or research.
- Professor Brendan McAndrew (IoA)
- Professor Herve Miguad (IoA)
- Dr David Penman (IoA)
- Dr Tom Hansen (IMR)
- Dr Julien Bobe (INRA)
- Dr Catherine Labbe (INRA)
The course will be based in IoA, University of Stirling, Stirling, Scotland, and will use the on-campus tropical fish facilities and the coldwater facilities at the Buckieburn laboratory and Howietoun Fish Farm.
18-22 November 2013. Four full days, including an evening programme.
Course attendance is FREE, thanks to EC FP7 funding. Participants are expected to pay for their own travel, subsistence and accommodation.
25 people (CV & description of motivation for wanting to attend the course may be used as selection criteria).
LANGUAGE OF INSTRUCTION AND MATERIAL