In this project, INRAE, the main institute for agronomic research in France, puts forward four installations for TNA.
1) INRAE-PEIMA (Experimental Fish Culture Station of Monts D’Arrée)
2) INRAE St Pée (Fish Nutrition Farms and platform)
3) INRAE-IERP (Fish Infectiology Platform)
4) INRAE-LPGP (Fish Physiology and Genomics Facility)
Name of the infrastructure: INRAE-LPGP (Fish physiology and Genomics Facility)
Location: Rennes, FRANCE
Website address: https://www6.rennes.inrae.fr/lpgp_eng/
Contact: Amélie Patinote (firstname.lastname@example.org); Jean-Charles Gabillard (jean-Charles.email@example.com)
The LPGP fish facility is an indoor structure based on recirculation systems which allows to control all the rearing parameters. The LPGP fish facility has the required level of biological safety for rearing genome-edited fish (trout, zebrafish, medaka, pike..) over a complete life cycle. It is equipped with:
• 20 independent recirculating water systems in 10 rooms (500m2).
• A quarantine room.
• A behavioural study room with camera on each tank.
• More than 100 large tanks for trout and 1,000 small tanks for model species.
• Room dedicated to eggs microinjection for production of transgenic or genome edited fish.
Four full-time permanent technical staff work on site, highly skilled in fish protocols in genome edition, physiology (reproduction, growth), and welfare.
Services currently offered by the infrastructure
The LPGP fish facility offers an array of services:
• INRAE LPGP proposes the production of transgenic or genome edited trout (GET service) that includes egg injection, genotyping and fish rearing up to reproductive stage. INRAE LPGP will train the users to genotyping and microinjection.
• The possibility to perform germ cell grafting (GCGraft service) into recipient embryos and the rearing of the surrogate fish up to maturation.
Modality of access
INRAE-LPGP fish facility will carry out experiments for potential users and provide physical access to it facility during crucial periods of the running experiments. As the standard procedures and the general maintenance will be carried out by trained and experienced staff, each user is expected to stay 5 days, for sampling and phenotyping of mutated trout (GET service) or grafted trout (GCGraft service). LPGP offers access to carry out fish trials with all tank types and water qualities available at the premises. Access will comprise the use of tanks including maintenance, water supply, daily feeding and husbandry of fish, manipulation, and sampling of fish. Access to all dry laboratory facilities and other infrastructural, logistical, technical, and scientific support to external users is offered, as well as accommodation and office access with PC and international phone, fax and internet communications. LPGP provides standardized experimental protocols, documentation of results, and appropriate sampling and conservation of samples.
Unit of access
The unit of access is defined as one tank/week. One typical access consists of 52 units of access (one tank for one year). One unit of access includes the injection of 400 trout eggs, genotyping and rearing until one year (F0). There are 104 units of access are allocated over the life of the project. Included in the units of access are monitoring of water quality parameters (flow, temperature, O2, CO2, pH), sanitary costs if needed (veterinary and treatment), sanitary safety, oxygen, et